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  • Title: [Primary research about reconstruction of cornea in three-dimensional collagen gel in vitro].
    Author: Chen J, Zhang S, Guo L, Zheng H, Lin J, Zheng J.
    Journal: Zhonghua Yan Ke Za Zhi; 2001 Jul; 37(4):244-7. PubMed ID: 11864428.
    Abstract:
    OBJECTIVE: To research the reconstruction of cornea in three-dimensional culture. METHODS: Using primary cultured corneal epithelial cells, stromal cells and endothelial cells rebuilt a corneal structure in three-dimensional collagen gel reconstruction system. The artificial corneal transparency was examined by a slit-lamp microscope. The biological characters of cells were tested by routine pathologic and transmission electron microscopic technology. RESULTS: The reconstructed corneal tissue formed three layers. The epithelial cell layer was two or three cell thickness. The endothelial cells formed a single layer. The stromal cells were diffuse in distribution in the collagen gel. The cells had proliferative ability and capability of producing collagen. CONCLUSION: The regular and transparent corneal structure can be rebuilt by tissue engineering technology.
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