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  • Title: [Detection and proliferation of hemorrhagic fever virus in chigger mites].
    Author: Zhang Y, Zhu J, Tang J, Li X, Wu G.
    Journal: Zhonghua Yu Fang Yi Xue Za Zhi; 1999 Mar; 33(2):98-100. PubMed ID: 11864463.
    Abstract:
    OBJECTIVE: To study the impact of Leptotromhidium scutellaris as a vector in transmission of hemorrhagic fever. METHODS: Reverse transcription-polymerase chain reaction (PCR) and in situ hybridization technique were used to detect RNA of hemorrhagic fever with renal syndrome virus (HFRSV). TCID(50)/ml of HFRSV were titrated for larva and nymph of chigger mites periodically. RESULTS: RNA of HFRSV was detected in the tissues of chigger mites, such as ovary cells, etc. by PCR and in situ hybridization. Titration of TCID(50)/ml showed that HFRSV could be transmitted transstadially and proliferated in chigger mites. CONCLUSION: It provides direct evidence at molecular level for the role of chigger mite as a vector in transmission of HFRSV with theoretical and practical importance in prevention of HFRS.
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