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  • Title: [Zinc promotes proliferation and differentiation of osteoblast in rats in vitro].
    Author: Cen X, Wang R, Wu Z.
    Journal: Zhonghua Yu Fang Yi Xue Za Zhi; 1999 Jul; 33(4):221-3. PubMed ID: 11864482.
    Abstract:
    OBJECTIVE: Effect of zinc on proliferation and differentiation of osteoblast in rats was studied in vitro. METHODS: Osteoblasts were isolated from the rat calvaria and subcultured in DMEM media. Rats were divided into four groups in the study, with administration of 0 micromol/L (as control), 10 micromol/L, 25 micromol/L and 50 micromol/L of Zn(2+), respectively. DNA synthesis at varied time points was determined with incorporation of (3)H-thymidine, and cell cycle was analyzed by flow cytometry. Synthesis of collagen was measured with incorporation of (3)H-proline. Activity of alkaline phosphatase (AKP) and osteocalcin content were measured by enzyme dynamics method and radioimmunoassay, respectively. RESULTS: Incorporation of (3)H-thymidine in the three groups with different zinc-supplement was significantly higher than that in the control group at each time point, and supplement with 25 micromol/L and 50 micromol/L of Zn(2+) could promote the transition of cell cycle from phases G(0)/G(1) to S phase. Syntheses of collagen and osteocalcin and activity of AKP in the groups with zinc-supplement were greater, as compared with those in the control group. CONCLUSION: Zinc could promote proliferation and differentiation of the rat osteoblast in vitro.
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