These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Apoptosis of HL60 cells induced by inhibition of bcl-2 with antisense phosphorothioate oligodeoxynucleotides].
    Author: Lin Y, Lu L, Chen Y.
    Journal: Zhonghua Bing Li Xue Za Zhi; 1999 Aug; 28(4):268-71. PubMed ID: 11869536.
    Abstract:
    OBJECTIVE: To observe the effects of different bcl-2 antisense phosphorothioate oligodeoxynucleotide (ASPO) concentrations in inducing apoptosis of HL60 cells. METHODS: Signs of apoptotic cells were detected by acridine orange staining, transmission electron microscope, flow cytometry and DNA fragment electrophoresis. RESULTS: In presence of ASPO, the apoptosis of HL60 cells could be induced after 24 hours of incubation. The apoptosis rate at ASPO 5, 10 and 20 micromol/L were (9.67 +/- 2.16)%, (18.25 +/- 3.01)% and (23.42 +/- 2.75)% respectively. There was a statistically significant increase in apoptosis as the dose of ASPO increased. After incubation for 72 hours, the apoptotic rates of the 3 different ASPO concentration groups were (9.58 +/- 2.96)%, (29.58 +/- 4.04)% and (36.33 +/- 3.66)% respectively, of which the apoptic rate of HL60 cells incubated with ASPO 5 micromol/L was highest at 48 hours. CONCLUSION: Bcl-2 ASPO can specifically induce apoptosis of HL60 cells. Its effect depends upon the concentration of ASPO.
    [Abstract] [Full Text] [Related] [New Search]