These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Quantitative determination of serum bile acids using a purified 3 alpha-hydroxysteroid dehydrogenase.
    Author: Fausa O.
    Journal: Scand J Gastroenterol; 1975; 10(7):747-52. PubMed ID: 1188308.
    Abstract:
    A specific, simple, and reliable method for the quantitative determination of 3 alpha-hydroxy bile acids in serum in described. It is based on the specific enzymatic conversion of the 3alpha-hydroxyl group of the steroid molecule using a highly purified 3alpha-hydroxysteroid dehydrogenase and fluorimetric determination of NADH. A liquid-solid extraction employing Amberlite XAD-2 has been used. Overall recovery of the major human bile acids from normal and jaundiced serum averaged 91.2%. The range of normal values for serum bile acids was found to be 0.9-6.3 mumoles/1 (mean 3.0).
    [Abstract] [Full Text] [Related] [New Search]