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  • Title: Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis.
    Author: Rastogi N, Venkitasubramanian TA.
    Journal: J Gen Microbiol; 1979 Dec; 115(2):517-21. PubMed ID: 119034.
    Abstract:
    Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).
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