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  • Title: A study of the lecithin/sphingomyelin ratio of amniotic fluid.
    Author: Gebhardt DO, Beintema A, de Rooij RE, Wildeboer FN, Merkus JM.
    Journal: Clin Chim Acta; 1975 Oct 15; 64(2):133-42. PubMed ID: 1191385.
    Abstract:
    1. There is a significant correlation between lecithin/sphingomyelin (L/S) ratios based on densitometry (L/S)D and L/S ratios based on phosphorus determinations ((L/S)P). 2. The fetal lung is mature when the (L/S)D, determined according to Verhoeven, A.G.J. and Merkus, H.M.W.M. (1974) Clin. Chim. Acta 53, 229--232, is 1.2. This value is equivalent to an (L/S)P of 1.8. 3. The acetone precipitation procedure, introduced by Gluck, L., Kulovich, M.V., Borer, R.C. and Keidel, W.N. (1974) Am. J. Obstet. Gynecol. 120, 142--155, is a necessary step for isolating surface-active lecithin. 4. Standardization of the (L/S)D test is feasible and should permit different laboratories to use the same transition point.
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