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  • Title: Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures.
    Author: Maes N, Magdalena J, Rottiers S, De Gheldre Y, Struelens MJ.
    Journal: J Clin Microbiol; 2002 Apr; 40(4):1514-7. PubMed ID: 11923385.
    Abstract:
    A triplex PCR targeting the 16S rRNA, mecA, and nuc genes was developed for identification of staphylococci and detection of methicillin resistance. After validation of the assay with a collection of strains of staphylococci and enterococci (n = 169), the assay was evaluated with cultures of blood with gram-positive cocci from 40 patients. Accurate results were obtained for 59 (98%) of 61 cultures within 6 h of growth detection.
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