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  • Title: [Validation of the HPLC method in the determination of dioxopromethazine and phenylephrine in eye drops].
    Author: Hudecová T, Hatrík S, Zimová N, Havránek E.
    Journal: Ceska Slov Farm; 2002 Mar; 51(2):91-5. PubMed ID: 11928283.
    Abstract:
    The present paper introduces a rapid HPLC method for the determination of dioxopromethazine and phenylephrine in eye drops. The method uses a modified C18 stationary phase optimized for the separation of basic compounds and a methanol/1.5 mM phosphoric acid (60/40 v/v, pH 3.02) mobile phase. The flow rate is set to 2 ml/min, sample volume 20 microliters, and compounds are detected at 275 nm. Prior to analysis, the eye drops are diluted with water in a ratio of 1:50. The elaborated HPLC method and the chromatographic system were validated according to the procedure for the validation of chromatographic systems and methods.
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