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  • Title: Anti-inflammatory effect of recombinant human superoxide dismutase in rats and mice and its mechanism.
    Author: Zhang Y, Wang JZ, Wu YJ, Li WG.
    Journal: Acta Pharmacol Sin; 2002 May; 23(5):439-44. PubMed ID: 11978194.
    Abstract:
    AIM: To investigate the anti-inflammatory effects and mechanism of recombinant human superoxide dismutase (rhSOD). METHODS: Inflammation models such as croton oil-induced ear swelling and carrageenan-induced hind paw edema in mice and rats were prepared. The nitric oxide synthase (NOS ) activity was measured by NADPH-diaphoras stain assay, N-acetyl-beta-D-glucosaminidase (beta-NAG) activity by spectrophotography, malondialdehyde (MDA) content by thiobarbituric acid (TBA) fluorescence technique, and interleukin-1beta (IL -1beta), tumor necrosis factor alpha (TNF alpha), and IL-8 content by radioimmunoassay (RIA). RESULTS: rhSOD 20-80 mg/kg ip, 40-80 mg/kg im, and 80 mg/kg ip significantly inhibited carrageenan-induced paw edema in rats, croton oil-induced ear swelling in mice, and carrageenan-induced hind paw edema in mice, respectively. In the rat arthritis induced by carrageenan, rhSOD 40 mg/kg reduced MDA content in inflamed paws, inhibited NOS activity, and lowered the content of IL-1beta and TNFalpha in exudate significantly. The inhibitory effect of rhSOD 40 mg/kg ip on IL-1beta production was more evident than that of dexamethasone 2 mg/kg ip. Also rhSOD obviously inhibited neutrophil infiltration; However, rhSOD had no effect on beta-NAG activity in exudate. CONCLUSION: rhSOD has anti-inflammatory effect on experimental inflammation in rats and mice, and its mechanisms are relevant to oxygen free radical scavenging, anti-lipid peroxidation, inhibition of neutrophil infiltration, and formation of inflammatory cytokines.
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