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  • Title: [Immobilization of highly purified cytochrome P-450 from microsomes of rabbit liver].
    Author: Eremin AN, Usanov SA, Mterlitsa DI.
    Journal: Prikl Biokhim Mikrobiol; 1979; 15(6):861-8. PubMed ID: 119962.
    Abstract:
    Highly purified cytochrome P-450 from microsomes of the rabbit liver was immobilized on albumin modified Bio-gel P-300 using the glutaraldehyde method. The immobilized enzyme had a higher stability in storage and thermal stability than the soluble enzyme and retained catalytic activity toward cumene hydroperoxide-dependent aniline hydroxylation. Kinetic characteristics of cumene hydroperoxide-dependent aniline oxidation by solubilized and immobilized enzymes were compared.
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