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  • Title: Pollination modulates expression of the PPAL gene, a pistil-specific beta-expansin.
    Author: Pezzotti M, Feron R, Mariani C.
    Journal: Plant Mol Biol; 2002 May; 49(2):187-97. PubMed ID: 11999374.
    Abstract:
    Using differential screening we isolated a pistil-specific cDNA clone corresponding to a 1.2 kb mRNA and encoding a 32.5 kDa protein. The amino acid sequence shared similarity with that of group-I grass pollen allergens, which are known to have expansin activity. This clone, which later showed to share homology also with beta-expansins, was named PPAL. The PPAL mRNA was specifically expressed in the secretory zone of the stigma and in the epidermal layer of the placenta. The accumulation level of the transcript increased during pollination, and the protein was secreted in the stigmatic exudate of the tobacco flower. We suggest here that PPAL is a new expansin, acting as a cell-wall-loosening agent during pollination.
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