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  • Title: In vitro mineralization of a glass-ceramic of the MgO-3CaO x P2O5-SiO2 system: wettability studies.
    Author: Serro AP, Fernandes AC, Saramago B, Fernandes MH.
    Journal: J Biomed Mater Res; 2002 Jul; 61(1):99-108. PubMed ID: 12001252.
    Abstract:
    The calcium phosphate deposition on the surface of a bioactive glass-ceramic of the MgO-3CaO. P(2)O(5)-SiO(2) system during a 1-week immersion in biological model fluids, was investigated through wettability studies. Two model fluids with chemical composition similar to the human blood plasma were tested: Hanks' balanced salt solution (HBSS) and a simulated body fluid (SBF) with a higher calcium content. The effect of the presence of albumin, both in solution or previously adsorbed to the surface, was assessed. The behavior observed using two SBF solutions prepared with and without TRIS buffer was compared. The surface free energy of the glass-ceramic samples was determined, before and after immersion, and dynamic contact angle analysis was performed using the biological model fluids as scanning liquids. Scanning electron microscope observations and x-ray photoelectron spectroscopy (XPS) analysis were performed to complement the wettability studies. The experimental results led to the conclusion that the precipitation of a calcium phosphate film in HBSS occurred mainly when the immersion solution was renewed daily or in the presence of adsorbed albumin. In SBF, the addition of TRIS buffer seemed to inhibit the formation of the calcium phosphate film.
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