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  • Title: Direct fluorescence polarization immunoassay.
    Author: Kobayashi Y, Miyai K, Tsubota N, Watanabe F.
    Journal: Steroids; 1979 Dec; 34(7):829-34. PubMed ID: 120042.
    Abstract:
    Direct fluorescence polarization immunoassay of serum cortisol was established. Non-specific binding of fluorescent-labelled cortisol to serum proteins was successively eliminated by sodium dodecyl sulfate. The minimal amount of cortisol detected was 0.1 ng/tube and serum concentration of 1.0 microgram/dl to 100 microgram/dl of cortisol could be measured. This fluorescence polarization immunoassay satisfied the standard criteria of accuracy and precision. The values correlated well with those obtained by radioimmunoassay.
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