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  • Title: Oxacillin susceptibility testing of coagulase-negative staphylococci using the disk diffusion method and the Vitek GPS-105 card.
    Author: Woods W, Ramotar K, Lem P, Toye B.
    Journal: Diagn Microbiol Infect Dis; 2002 Apr; 42(4):291-4. PubMed ID: 12007449.
    Abstract:
    One hundred and ninety-three isolates of coagulase-negative staphylococci (CoNS) were tested against oxacillin by agar dilution, disk diffusion, and Vitek (GPS-105 card), and the presence of the mecA gene determined by multiplex PCR. The results obtained by all testing methods were in agreement for 190 isolates. Two mecA-negative isolates (S. lugdunensis and S. haemolyticus) had MICs of < or = 0.25 microg/ml by agar dilution and Vitek but were resistant by disk diffusion. One mecA-positive isolate was resistant by Vitek and disk diffusion but had an agar dilution MIC of < or = 0.25 microg/ml. For the species of CoNS tested, oxacillin susceptibility results obtained with the Vitek GPS-105 card and disk diffusion correlated well with results obtained by National Committee for Clinical Laboratory Standards agar dilution and with the presence of the mecA gene.
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