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  • Title: Insulin induces PFKFB3 gene expression in HT29 human colon adenocarcinoma cells.
    Author: Riera L, Manzano A, Navarro-Sabaté A, Perales JC, Bartrons R.
    Journal: Biochim Biophys Acta; 2002 Apr 03; 1589(2):89-92. PubMed ID: 12007784.
    Abstract:
    Fructose 2,6-bisphosphate is present at high concentrations in many established lines of transformed cells. It plays a key role in the maintenance of a high glycolytic rate by coupling hormonal and growth factor signals with metabolic demand. The concentration of fructose 2,6-bisphosphate is controlled by the activity of the homodimeric bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2). We report here the PFKFB-3 gene expression control by insulin in the human colon adenocarcinoma HT29 cell line. The incubation of these cells with 1 microM insulin resulted in an increase in the PFK-2 mRNA level after 6 h of treatment, this effect being blocked by actinomycin D. Furthermore, insulin induced ubiquitous PFK-2 protein levels, that were evident after a lag of 3 h and could be inhibited by incubation with cycloheximide.
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