These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Mapping nucleotide binding site of calcium ATPase with IR spectroscopy: effects of ATP gamma-phosphate binding.
    Author: Liu M, Barth A.
    Journal: Biopolymers; 2002; 67(4-5):267-70. PubMed ID: 12012444.
    Abstract:
    The changes in the IR spectra of the sarcoplasmic reticulum Ca2+-ATPase upon nucleotide binding are recorded in H2O at 1 degrees C in different buffers [imidazole, methylimidazole, 3-(N-morpholino)propanesulfonic acid, and phosphate] at different pH values (pH 6.5-7.8). The difference spectra of nucleotide binding are sensitive to the composition of the solvent. With methylimidazole at pH 7.5 providing the largest binding-induced signals, the effects of gamma-phosphate binding are investigated using ATP, ADP, and beta,gamma-iminoadenosine 5'-triphosphate. The gamma-phosphate contributes approximately 20% to the conformational change seen by IR spectroscopy and affects the beta-sheet structures. The IR experiments also reveal the known affinity difference between ADP and ATP.
    [Abstract] [Full Text] [Related] [New Search]