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  • Title: Effects of two 10% peroxide carbamide bleaching agents on dentin microhardness at different time intervals.
    Author: de Freitas PM, Basting RT, Rodrigues JA, Serra MC.
    Journal: Quintessence Int; 2002 May; 33(5):370-5. PubMed ID: 12014167.
    Abstract:
    OBJECTIVE: The purpose of this in vitro study was to evaluate the microhardness of human dentin exposed to two 10% carbamide peroxide agents at different bleaching times. METHOD AND MATERIALS: Opalescence 10% and Rembrandt 10% were tested. A placebo agent was used as a control group. The bleaching and placebo agents were applied to the surface of human dentin fragments for 8 hours and then stored in individual receptacles with artificial saliva for the remaining 16 hours each day. Microhardness testing was performed at baseline, after 8 hours, 7, 14, 21, 28, 35, and 42 days of treatment, and 7 and 14 days posttreatment. RESULTS: Analysis of Variance and the Tukey test revealed significant differences in microhardness values for dentin treated with the agents within each time interval. There was a decrease in the microhardness values of dentin for both bleaching agents after 8 hours of treatment. Fourteen days after the completion of treatment, the microhardness values for dentinal surfaces treated with either Opalescence or Rembrandt reached the baseline values; the dentinal surfaces treated with a placebo exhibited an increase in microhardness values posttreatment. CONCLUSION: Ten percent carbamide peroxide bleaching agents decreased dentinal microhardness over time, but after 14 days in artificial saliva storage at the completion of treatment, the baseline microhardness values were recovered.
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