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  • Title: [Construction and expression of a vector containing protein transduction domain and bcr/abl fusion gene].
    Author: Liang Y, Sun Q, Jiang S, Wang J, Wu R, Chen P, Liu L, Han H.
    Journal: Zhonghua Xue Ye Xue Za Zhi; 2002 Jan; 23(1):5-8. PubMed ID: 12015079.
    Abstract:
    OBJECTIVE: To construct a vector containing protein transduction domain (PTD) and bcr/abl fusion gene of chronic myelogenous leukemia and express PTD-bcr/abl fusion protein in E. Coli. METHODS: DNA fragment encoding PTD was synthesized and fused to PCR-amplified bcr/abl gene fragment, then inserted into plasmid pET-16b to get the expression vector pEPb containing PTD-bcr/abl fusion gene, which was transfected and expressed in E. Coli LB21. PTD-bcr/abl fusion protein was purified by affinity chromatography. RESULTS: 523 bp bcr/abl fusion gene was effectively amplified. The PTD-bcr/abl gene sequencing showed the same sequence as scheduled. The fusion peptide was successfully expressed in E. Coli and purified. CONCLUSION: The results may provide a new PTD-bcr/abl fusion peptide for the immunotherapy of CML.
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