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  • Title: Excitation and desensitization of mouse rod photoreceptors in vivo following bright adapting light.
    Author: Kang Derwent JJ, Qtaishat NM, Pepperberg DR.
    Journal: J Physiol; 2002 May 15; 541(Pt 1):201-18. PubMed ID: 12015430.
    Abstract:
    Electroretinographic (ERG) methods were used to determine response properties of mouse rod photoreceptors in vivo following adapting illumination that produced a significant extent of rhodopsin bleaching. Bleaching levels prevailing at approximately 10 min and approximately 20 min after the adapting exposure were on average 14 % and 9 %, respectively, based on the analysis of visual cycle retinoids in the eye tissues. Recovery of the rod response to the adapting light was monitored by analysing the ERG a-wave response to a bright probe flash presented at varying times during dark adaptation. A paired-flash procedure, in which the probe flash was presented at defined times after a weak test flash of fixed strength, was used to determine sensitivity of the rod response to the test flash. Recovery of the response to the adapting light was 80 % complete at 13.5 +/- 3.0 min (mean +/- S.D.; n = 7) after adapting light offset. The adapting light caused prolonged desensitization of the weak-flash response derived from paired-flash data. By comparison with results obtained in the absence of the adapting exposure, desensitization determined with a test-probe interval of 80 ms was ~fourfold after 5 min of dark adaptation and approximately twofold after 20 min. The results indicate, for mouse rods in vivo, that the time scale for recovery of weak-flash sensitivity substantially exceeds that for the recovery of circulating current following significant rhodopsin bleaching. The lingering desensitization may reflect a reduced efficiency of signal transmission in the phototransduction cascade distinct from that due to residual excitation.
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