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  • Title: Optimizing ammonia processing conditions to enhance susceptibility of legumes to fiber hydrolysis: alfalfa.
    Author: Ferrer A, Byers FM, Sulbarán-de-Ferrer B, Dale BE, Aiello C.
    Journal: Appl Biochem Biotechnol; 2002; 98-100():123-34. PubMed ID: 12018242.
    Abstract:
    An ammonia process was applied at several ammonia loadings, moisture contents, temperatures, and dwell times. A cellulase loading of 5 FPU/g dry matter and a 24 h incubation time were used to produce the sugars, which were measured as reducing sugars and by HPLC. Optimal processing conditions caused a 76% of theoretical yield (2.9-fold above untreated). Cellulose and hemicellulose conversions were 68 and 85% (vs 38 and 34% in untreated, respectively). The short hydrolysis time and relatively low enzyme loading suggests great potential to produce sugars from alfalfa.
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