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  • Title: The catalytic pathway of horseradish peroxidase at high resolution.
    Author: Berglund GI, Carlsson GH, Smith AT, Szöke H, Henriksen A, Hajdu J.
    Journal: Nature; 2002 May 23; 417(6887):463-8. PubMed ID: 12024218.
    Abstract:
    A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
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