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  • Title: [Quantitative determination of N-terminal amino acids in fibrin by the Edman degradation method using thin-layer chromatography].
    Author: Luhovskoy EV, Derzska SH, Hoholynska HK, Lytvynova LM.
    Journal: Ukr Biokhim Zh; 1975; 47(6):760-7. PubMed ID: 1202713.
    Abstract:
    Separation and identification of phenylthiohydantoin derivatives (PTH) of 19 amino acids usually found in proteins was performed by thin-layer chromatography of "silufol" plates. Conditions of PTH quantitation were found at which a 90% extraction of PTH from silicagel was attained. A N-terminal analytical procedure for fibrin is elaborated, by which a high yield of N-terminal amino acids is obtained.
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