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  • Title: Rapid and sensitive determination of nalmefene in human plasma by gas chromatography-mass spectrometry.
    Author: Xie S, Suckow RF, Mason BJ, Allen D, Cooper TB.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2002 Jun 25; 773(2):143-9. PubMed ID: 12031840.
    Abstract:
    A rapid gas chromatography-mass spectrometric method for the determination of nalmefene in human plasma is described. The procedure involves protein precipitation, extraction with ethanol-chloroform mixture and derivatization with pentafluropropionic anhydride. The deuterated analog of nalmefene, 6beta-naltrexol-d(7), was used as the internal standard. Quantitation was achieved on a HP-1 column (12 mx0.2 mm I.D.) with negative chemical ionization (NCI) using methane:ammonia (95:5) as the reagent gas. The standard curves were fitted using a quadratic equation with the curve encompassing a range of 0.5 to 200 ng/ml, and the intra- and inter-assay variations for three different nalmefene levels were less than 10% throughout. The limit of quantitation was found to be 0.5 ng/ml. The method described is highly specific and reproducible, and could also be applied for the determination of naltrexone and 6beta-naltrexol. Application of the method to actual human plasma samples is demonstrated.
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