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  • Title: Metabolism of [4-14C] testosterone in the rat uterus in vitro.
    Author: Hoffmann U, Maass H, Lisboa BP.
    Journal: Eur J Biochem; 1975 Nov 01; 59(1):305-12. PubMed ID: 1204615.
    Abstract:
    In view of the uterine action of androgens we have investigated in vitro the metabolism of [4-14C]-testosterone in uterine tissue of ovariectomized rats. After purification of the extracts on Amberlite XAD-2 the metabolites have been isolated by gel. Five metabolites were isolated and identified during these incubation studies: 4-androstene 3,17-dione, 17beta-hydroxy-5alpha-androstan-3-one, 5 alpha-androstane-3alpha17beta-diol, 4-androstene-3 beta, 17beta-diol and 4-androstene-3alpha, 17beta-diol. Furthermore, two polar C19O3-metabolites and one isopolar to 5 alpha-androstane-3, 17-dione have also been detected. The metabolites were characterized by radioactive gas chromatogrphy, and determination of the relative specific activity in the eluates of Sephadex column chromatography. The identification of allylic alcohols was complemented by their oxidation to 4-androstene-3,17-dione. The present data show that activity of 17beta,3alpha- and 3beta-hydroxysteroid-oxidoreductase and 5alpha-ring-reductase are involved in the metabolism of testosterone in vitro in the rat uterus. The very low 5 alpha-reductase activity under the experimental conditions used in this work explains the formation of allylalcohols as the principal metabolites of testosterone in the rat uterus. The metabolism of 4-carbon-14-testosterone was investigated in the rat uterus in vitro. Purification and isolation of 5alpha-dihydrotestosterone, 4-androstene-3,17-dione, 5alpha-androstane-3alpha, 17beta diol were carried out with Amberlite XAD-2, gel column chromatography Sephadex LH 20, and thin-layer chromatography on silica gel. Radioactive gas chromatography was used for characterization and relative specific activity was determined by Sephadex column chromatography. Identification of allylic alcohols was complemented by their oxidation to 4-androstene-3,17-dione. These data reveal that 17beta,3alpha and 3beta-hydroxysteroid-oxidoreductase and 5alpha-ring-reductase activities are involved in testosterone metabolism in vitro in the rat uterus. The low 5alpha-reductase activity under these conditions explains the formation of allylacohols as the principal metabolites of testosterone.
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