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  • Title: [Dystrophin detection by immunofluorescent technique for diagnosing muscular dystrophy].
    Author: Wang X, Xie Y, Zhang C, Liu Z.
    Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 2002 Jun; 19(3):239-42. PubMed ID: 12048687.
    Abstract:
    OBJECTIVE: To establish a specific technique for diagnosing and classifying Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), facioscapulohumeral muscular dystrophy (FSHD) and neurologic dystrophy. METHODS: Forty-seven cases were detected by immunofluorescence technique for analyzing dystrophin located in skeletal muscle cell membrane with the use of mouse monoclonal antibodies, goat and rabbit polyclonal antibodies. RESULTS: The normal individuals showed ringed positive staining stripe around muscle fibers. Negative result of staining was seen in 16 DMD patients. Eleven BMD patients had discontinuous or a patchy positive staining pattern, and all of 10 FSHD and 10 neurological amyotrophic patients showed positive dystrophin staining. CONCLUSION: Detecting dystrophin in the skeletal muscle cell membrane of muscular patients is an efficient technique for diagnosing and classifying various types of muscular dystrophy.
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