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  • Title: NMR evidence for different conformations of the bioactive region of rat CCK-8 and CCK-58.
    Author: Keire DA, Solomon TE, Reeve JR.
    Journal: Biochem Biophys Res Commun; 2002 May 10; 293(3):1014-20. PubMed ID: 12051761.
    Abstract:
    Sulfated CCK-58 and CCK-8 have identical bioactive C-terminal primary sequences but distinct C-terminal solution structures and different bioactivities. To examine structural differences in greater detail, rat CCK-58 and -8 were synthesized with isotopic enrichment of C-terminal residues with (15)N at alpha-amino nitrogens. Proton and nitrogen chemical shift assignments of peptide solutions were obtained by homo- and heteronuclear NMR methods. These data show that the tertiary structure ensembles of C-terminal CCK-8 and CCK-58 differ significantly. Thus, distinct solution conformations may explain differences in CCK(A) and CCK(B) receptor interactions of large and small molecular forms of CCK.
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