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  • Title: [Sample preparation for electron microscope with tissues fixed with formalin and embedded in paraffin].
    Author: Jing J.
    Journal: Hunan Yi Ke Da Xue Xue Bao; 1999; 24(6):581-2. PubMed ID: 12080729.
    Abstract:
    A method of sample preparation for electron microscope is introduced. After paraffin-embedded tissues, fresh and fixed on time, were deparaffined, the tissue were fixed in 4% glutaraldehyde for 24 hours, in 1% osmic acid for 1-2 hours, and dehydrated with acetone and embedded in Epon812; then they were cut into ultrathin sections. By this method, better ultrastructure of paraffin-embedded tissues was obtained, and the method could satisfy the demand for observation under electron microscope. In addition, some important proceedings were also discussed in the paper.
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