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  • Title: Amiodarone induces apoptosis in L-132 human lung epithelial cell line.
    Author: Choi IS, Kim BS, Cho KS, Park JC, Jang MH, Shin MC, Jung SB, Chung JH, Kim CJ.
    Journal: Toxicol Lett; 2002 Jun 07; 132(1):47-55. PubMed ID: 12084619.
    Abstract:
    To investigate whether amiodarone induces apoptosis in cells of the L-132 human lung epithelial cell line, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, 4,6-diamidino-2-phenylindole staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction, and casapse-3 enzyme assay were performed. Through morphological and biochemical analyses, it was demonstrated that L-132 cells treated with amiodarone exhibit several features of apoptosis. In addition, it was shown that amiodarone increases the mRNA levels of bax and caspase-3. Based on the results, amiodarone appears to activate specific intracellular death-related pathways, including possibly the bax-dependent caspase-3 activation pathway, and thus induce apoptosis in human lung epithelial cells.
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