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  • Title: Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryos.
    Author: Ikeda-Iwai M, Satoh S, Kamada H.
    Journal: J Exp Bot; 2002 Jul; 53(374):1575-80. PubMed ID: 12096096.
    Abstract:
    Somatic embryogenesis is an example of totipotency and is used as a model system for studying embryogenesis. A reproducible tissue culture system was established for the large-scale induction of Arabidopsis somatic embryos. The method allows maintenance of high embryogenic competence over a one-year period. Using this tissue culture system, the expression of embryo-specific genes (ABI3, LEC1, FUS3) was detected in embryogenic cells and somatic embryos. Exogenous application of abscisic acid enhanced the expression of some late-embryogenesis-abundant (LEA) protein genes in somatic embryos. The experiments show that the method can be used to obtain sufficient amounts of embryogenic material for basic molecular analyses.
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