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  • Title: [Utilization of serological methods in the retrospective diagnosis of cholera and in detecting vibrion carriers].
    Author: Bunin KV, Firsanov VI, Kravtsov EG, Nechaeva IP, Efimov DD.
    Journal: Zh Mikrobiol Epidemiol Immunobiol; 1975 Oct; (10):98-101. PubMed ID: 1210898.
    Abstract:
    Sensitivity and specificity of the three serological methods were studied comparatively: the vibriocidal test, the reaction of bacterial agglutination and of indirect hemagglutination, with the use of erythrocytes sensitized with the vibrio lyzate, cholera species O-antigen and cholerogen. Investigations were conducted with the blood sera of cholera patients, vibrio carriers and contacts. Vibriocidal test proved to be the most sensitive; its data correlated with the results of bacterial agglutination and indirect hemagglutination with erythrocytes, sensitized with the lysate of the vibrios and the cholera O-antigen. None of the used serological methods provided a 100% coincidence with the results of bacteriological analysis. The frequency of detection of anticholera antibodies decreased in the following order: cholera patients, vibrio carriers, contacts.
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