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  • Title: A sieving method for collecting the metacercariae of trematode parasites from freshwater fish.
    Author: Fan PC, Wu CC, Huang P, Yen CW.
    Journal: Southeast Asian J Trop Med Public Health; 2002 Mar; 33(1):23-4. PubMed ID: 12118453.
    Abstract:
    This study describes a sieving method for the collection of metacercariae from frozen (-20 degrees C) freshwater fish. Digested fish tissue is filtered through a series of sieves; the crude filtrate is then centrifuged. Centrifugation produces a sediment from which metacercariae can be removed. Half of the metracercariae that were obtained from the fish meat that had been frozen for 10 days (-20 degrees C) were dead; the other half were alive and some larvae were moving slowly.
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