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  • Title: An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles.
    Author: Vanholme B, De Meutter J, Tytgat T, Gheysen GD, Vanhoutte I, Gheysen GD.
    Journal: Parasitol Res; 2002 Aug; 88(8):731-3. PubMed ID: 12122430.
    Abstract:
    An optimized protocol is presented to visualize gene expression in the sedentary beet cyst nematode, Heterodera schachtii, by whole-mount in situ hybridization. Two different probes were used for genes with known expression pattern in other nematodes. Vacuum infiltration of the fixative significantly increased its efficiency and resulted in a nicely preserved morphology. Additional modifications were introduced to simplify and standardize the process.
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