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  • Title: K(+) current distribution in rat sub-epicardial ventricular myocytes.
    Author: Komukai K, Brette F, Yamanushi TT, Orchard CH.
    Journal: Pflugers Arch; 2002 Jul; 444(4):532-8. PubMed ID: 12136273.
    Abstract:
    In the rat ventricle, the transient outward K(+) current ( I(TO)) is carried by heteromeric channels composed of Kv4.2 and Kv4.3. However its distribution in the cell membrane is unclear: immunohistochemical studies of Kv4.2 distribution in the cardiac ventricular cell membrane have given equivocal results, and there are no corresponding studies of Kv4.3. We therefore used detubulated cardiac cells to investigate the functional distribution of I(TO) between the t-tubules and surface membrane. I(TO), the delayed rectifier ( I(K)), the inward rectifier ( I(K1)) and steady-state ( I(SS)) K(+) currents were monitored using the patch-clamp technique in control and formamide-treated (detubulated) cells from rat left ventricular sub-epicardium. Formamide treatment decreased cell capacitance by 20%, did not significantly change the density of I(TO), I(K) or I(K1) but decreased the density of I(SS) and L-type Ca current ( I(Ca)). These data suggest that I(TO), I(K), and I(K1) are uniformly distributed between the surface and t-tubule membranes, but that I(SS) and I(Ca) are concentrated in the t-tubules.
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