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  • Title: Molecular breeding of transgenic rice plants expressing a bacterial chlorocatechol dioxygenase gene.
    Author: Shimizu M, Kimura T, Koyama T, Suzuki K, Ogawa N, Miyashita K, Sakka K, Ohmiya K.
    Journal: Appl Environ Microbiol; 2002 Aug; 68(8):4061-6. PubMed ID: 12147507.
    Abstract:
    The cbnA gene encoding the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 was introduced into rice plants. The cbnA gene was expressed in transgenic rice plants under the control of a modified cauliflower mosaic virus 35S promoter. Western blot analysis using anti-CbnA protein indicated that the cbnA gene was expressed in leaf tissue, roots, culms, and seeds. Transgenic rice calluses expressing the cbnA gene converted 3-chlorocatechol to 2-chloromucote efficiently. Growth and morphology of the transgenic rice plants expressing the cbnA gene were not distinguished from those of control rice plants harboring only a Ti binary vector. It is thus possible to breed transgenic plants that degrade chloroaromatic compounds in soil and surface water.
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