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  • Title: [Nested coamplification polymerase chain reaction].
    Author: Xia Q, Zhang S, Yang Y, Wang J, Wang X, Ma Y, Sun Y.
    Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 2002 Aug; 19(4):347-9. PubMed ID: 12170477.
    Abstract:
    OBJECTIVE: To develop a simple and reliable method for intensifying the hybridization signals of gene chips. METHODS: The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip. RESULTS: With the use of EDTA and another FAM-labeled probe, the hybridization signals increased by 6 times or greater. CONCLUSION: Adding EDTA and another probe to the normal PCR products is a simple and efficient method to intensify the hybridization signal of chips.
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