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  • Title: Multilaboratory comparison of anaerobe susceptibility results using 3 different agar media.
    Author: Roe DE, Finegold SM, Citron DM, Goldstein EJ, Wexler HM, Rosenblatt JE, Cox ME, Jenkins SG, Hecht DW.
    Journal: Clin Infect Dis; 2002 Sep 01; 35(Suppl 1):S40-6. PubMed ID: 12173107.
    Abstract:
    A 5-laboratory study was performed that used the National Committee for Clinical Laboratory Standards (NCCLS) reference agar dilution method with 3 media formulations to determine whether the use of different media would affect minimum inhibitory concentration (MIC) results. Wilkins-Chalgren, Brucella-based blood agar (BRU), and Wilkins-Chalgren agar plus blood (WCB) and 6 antibiotics (clindamycin, cefoxitin, ceftizoxime, piperacillin, metronidazole, and trovafloxacin) were evaluated with 58 isolates. The MIC values were compared, and a significant correlation of >0.80 was demonstrated for all media and each antibiotic/organism group. The cumulative rate of errors for all antibiotics was 0.1%. These data indicate that a change in the NCCLS reference medium for testing of anaerobic bacteria susceptibility to either BRU or WCB will not affect the MIC results for the antibiotics and organisms evaluated.
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