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  • Title: Fluorescein-based pI markers for capillary isoelectric focusing with laser-induced fluorescence detection.
    Author: Slais K, Horká M, Novácková J, Friedl Z.
    Journal: Electrophoresis; 2002 Jun; 23(11):1682-8. PubMed ID: 12179988.
    Abstract:
    We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented.
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