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  • Title: Characterization of Habu thrombin-like enzyme (THLE) with a new n-terminal sequence from the venom of Trimeresurus flavoviridis (Habu).
    Author: Komori Y, Tanaka K, Nikai T.
    Journal: J Nat Toxins; 2002 Aug; 11(3):205-12. PubMed ID: 12182540.
    Abstract:
    A thrombin-like enzyme with a new amino-terminal sequence was isolated from the venom of Trimeresurus flavoviridis using Q-Sepharose, CM-Cellulose, and HW55 column chromatographies. Homogeneity was confirmed by the formation of a single band in polyacrylamide gel electrophoresis. The enzyme has a molecular weight of 29,000 Da. This thrombin-like enzyme was inhibited by p-amidinophenylmethanesulfonyl fluoride hydrochloride (p-APMSF), and dithiothreitol (DTT) suggesting that serines and disulfide bonds are involved in the expression of the enzyme's clotting activity. This thrombin-like enzyme hydrolyzes the Aalpha-chain and Bbeta-chain of bovine fibrinogen. The enzyme was stable to heat treatment.
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