These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Comparative study of three short-chain neurotoxins from the venom of Naja kaouthia (Yunnan, China).
    Author: Qiumin L, Qingxiong M, Dongsheng L, Shaowen Z, Yonghong J, Yuliang X, Wanyu W.
    Journal: J Nat Toxins; 2002 Aug; 11(3):221-9. PubMed ID: 12182542.
    Abstract:
    Three short-chain neurotoxins named NT-1, NT-II, and NT-III were purified from the venom of Naja kaouthia, a snake distributed throughout the south of Yunnan province, China, by a series of chromatographic steps, including an FPLC Resource S column. Their molecular weights, determined by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS, were 6952.19 Da, 6854.92 Da, and 6828.80 Da, respectively. NT-I consisted of 62 amino acid residues, and the other two consisted of 61 amino acid residues, including 8 cysteines. After hydrolysis by endoproteinase Glu-C, their primary sequences were determined. A test of their activities demonstrated that they effectively inhibited muscle contractions induced by electric stimulation. Furthermore, the extent of inhibition caused by NT-II and NT-III was less than that of NT-I. The IC50s were 0.04 microg/ml, 0.20 microg/ml, and 0.23 microg/ml for NT-I, NT-II, and NT-III, respectively. Compared with NT-II and NT-III, the higher activity of NT-I may be a result of the amino acid residue substitution Ile36 to Arg36.
    [Abstract] [Full Text] [Related] [New Search]