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  • Title: [Cloning and expression of the cecropin B-thanatin hybrid antimicrobial peptide in Escherichia coli].
    Author: Weng HB, Niu BL, Meng ZQ, Xu MK.
    Journal: Sheng Wu Gong Cheng Xue Bao; 2002 May; 18(3):352-5. PubMed ID: 12192873.
    Abstract:
    A 44-residue hybrid peptide (CB (1-24)-Arg-Ser-Tyr-Tan (4-21)) incorporating 1-24 residues of cecropin B (CB) and 4-21 residues of thanatin (Tan) was designed and constructed. The CB-Tan gene was cloned into expression plasmid pGEX-3X and expressed in E. coli BL21. The fusion protein was purified by affinity chromatography. After digested with enterokinase the gene product released with antibacterial activity and gave one band in Tricine-SDS-PAGE.
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