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  • Title: [A study on chemical composition of Gardenia sootepensis Hutch--quantitative determination of iridoid compounds by RP-HPLC].
    Author: Wang G, Zhao S, Chen D.
    Journal: Zhongguo Zhong Yao Za Zhi; 1999 Oct; 24(10):616-8, 639. PubMed ID: 12205960.
    Abstract:
    OBJECTIVE: To determine the iridoid contents in Gardenia sootepensis. METHODS: Using RP-HPLC with geniposidic acid, geniposide and scandoside methyl ether as standards. RESULT: According to the selected chromatographic conditions, the linear ranges of geniposidic acid, geniposide and scandoside methyl ether were 0.097-0.606 microgram, 0.0638-3.990 micrograms and 0.198-1.239 micrograms, respectively. The average recoveries of these three compounds were 100.0%, 99.9% and 100.1%, and RSD 1.23%, 1.38% and 1.53% (n = 5) respectively. CONCLUSION: The method is easy, rapid, and reproducible.
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