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  • Title: High performance liquid chromatographic determination of glucosamine in rat plasma.
    Author: Aghazadeh-Habashi A, Sattari S, Pasutto F, Jamali F.
    Journal: J Pharm Pharm Sci; 2002; 5(2):176-80. PubMed ID: 12207870.
    Abstract:
    PURPOSE: A high performance liquid chromatographic method was developed for the determination of glucosamine (GlcN) in rat plasma. METHOD: Internal standard, galactosamine, was added to 100 micro L of plasma containing GlcN followed by precipitation of plasma proteins with acetonitrile. Evaporation of the decanted supernatant solution was accelerated by the addition of methanol. GlcN was derivatized by addition of a solution containing 1-naphthyl isothiocyanate. Sample cleanup included passage through an anion exchange cartridge. Analysis was accomplished by injection of 0.1 mL of the sample solution into an isocratic HPLC system consisting of a C18 column, a mobile phase of acetonitrile: water: acetic acid: triethylamine (4.5: 95.5:0.1:0.05), a flow rate of 0.9 mL/min, and a UV detector set at 254 nm. RESULTS: Galactosamine and GlcN appeared 26 and 29 min post-injection, respectively. The assay was linear over the range of 1.25-400 micro g/mL (CV<10%) with a detection limit of 0.63 microg/mL and a limit of quantification of 1.25 microg/mL. The method was applied to the determination of GlcN in rat plasma after oral administration of 350 mg/kg of GlcN hydrochloride. CONCLUSION: The present assay is specific, sensitive, precise, and accurate and is suitable for pharmacokinetic studies.
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