These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Concentration gradient used in double-stranded DNA separation by capillary electrophoresis.
    Author: Liang D, Chu B.
    Journal: Electrophoresis; 2002 Aug; 23(16):2602-9. PubMed ID: 12210163.
    Abstract:
    Effects of concentration gradient on double-stranded DNA (dsDNA) separation by capillary electrophoresis are presented. By using a concentration gradient in the range between 0.8% and 3.2% for poly(N,N-dimethylacrylamide) (PDMA), the presence of a mesh-size gradient in the capillary could enhance the separation of larger size DNA fragments, better than that based on a single uniform concentration over the same capillary length. A decrease in the column length could make the gradient effect more obvious. An optimal capillary length could be achieved by using a judicious combination of the concentration gradient and the concentration range, yielding a maximum resolution for the system. The standard deviation of the migration time measured for each DNA fragment was less than 5% in ten continuous runs, suggesting that the gradient formed inside the column was quite stable.
    [Abstract] [Full Text] [Related] [New Search]