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  • Title: [The mechanism of macrophage apoptosis induced by lipopolysaccharide in mice].
    Author: Luo FJ, Xiao XZ, You JL, Wang YR, Luo ZY.
    Journal: Hunan Yi Ke Da Xue Xue Bao; 2000 Oct 28; 25(5):431-4. PubMed ID: 12212109.
    Abstract:
    Apoptosis is a major form of cell death, characterized morphologically by chromatin condensation and biochemically by endonuclease cleavage of DNA into oligonucleosomal fragments. We investigated apoptosis mechanism of peritoneal macrophage(M phi) induced by peritoneal injection of lipopolysaccharide(LPS) in mice. The results showed that: LPS induced the apoptosis of peritoneal M phi and concomitant decrease of phagocytosis(vs control group, P < 0.01), the concentration of NO2-/No3- in the peritoneal lavage fluid significantly increased after LPS injection; AG(inhibitor of iNOS) and PDTC(inhibitor of reactive oxygen species) prevented the apoptosis of M phi and reduced the concentration of NO2-/NO3- in the peritoneal lavage fluid. In vitro experiment, we found that AG and PDTC inhibited the apoptosis of M phi induced by IFN(100 U.ml-1) + LPS (10 micrograms.ml-1) by using DNA gel electrophoresis analysis. These evidences support that NO and active oxygen species may be involved in the apoptosis process of peritoneal M phi induced by LPS in mice.
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