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  • Title: [Spectrophotometric determination of copper in the hair of the patients with rheumatoid arthritis].
    Author: Cheng XY, Li ZH.
    Journal: Hunan Yi Ke Da Xue Xue Bao; 2000 Apr 28; 25(2):117-8. PubMed ID: 12212193.
    Abstract:
    A new color reaction of copper (II) with solochrome cyanine R was studied by spectrophotometry. The results were that in the pH6 of HAc-NaAc buffer solution solochrome cyanine R reacted with copper (II) to form a violet complex; the maximum absorption of the complex was at 565 nm and its apparent molar absorption coefficient was 8.25 x 10(5) L.mol-1.cm-1. Beer's Law was obeyed in the range of 0-0.48 microgram.ml-1. This method was used to determine copper levels in the hair samples of 5 normal persons and 5 rheumatoid arthritis patients. The results showed that the hair copper level of arthritis patients was significantly higher than that of normal persons (P < 0.01).
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