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  • Title: Single column approach for the liquid chromatographic separation of polar and non-polar glucosinolates from broccoli sprouts and seeds.
    Author: West L, Tsui I, Haas G.
    Journal: J Chromatogr A; 2002 Aug 09; 966(1-2):227-32. PubMed ID: 12214698.
    Abstract:
    Ion-pair and hydrophilic interaction chromatographies are considered to be complementary methods of choice for analyzing intact glucosinolates from broccoli. Ion-pair chromatography resolves non-polar glucosinolates, such as those containing indole moieties, while hydrophilic interaction chromatography is superior for separating polar glucosinolates, such as glucoraphanin and glucoiberin. Reversed-phase separations using hydrophilic endcapped C18-bonded silica and a 50 mM ammonium acetate-methanol gradient mobile phase resolve both polar and non-polar glucosinolates negating the need for switching columns.
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