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  • Title: Ca(2+) signaling and membrane potential in descending vasa recta pericytes and endothelia.
    Author: Rhinehart K, Zhang Z, Pallone TL.
    Journal: Am J Physiol Renal Physiol; 2002 Oct; 283(4):F852-60. PubMed ID: 12217877.
    Abstract:
    We devised a method for removal of pericytes from isolated descending vasa recta (DVR). After enzymatic digestion, aspiration of a descending vas rectum into a micropipette strips the pericytes from the abluminal surface. Pericytes and denuded endothelia can be recovered for separate study. Using fura 2-loaded preparations, we demonstrated that 10 nM angiotensin II (ANG II) elevates pericyte intracellular Ca(2+) concentration ([Ca(2+)](i)) and suppresses endothelial [Ca(2+)](i). The anion transport blocker probenecid helps retain fura 2 in the pericyte cytoplasm. DVR endothelia were accessed for membrane potential measurement by perforated-patch whole cell recording by using the pericyte-stripping technique and by turning nondigested vessels inside out with concentric micropipettes. By either method of access, 10 nM ANG II depolarized (n = 20) and 100 nM bradykinin hyperpolarized (n = 25) the endothelia. We conclude that isolated endothelia and pericytes remain functional for study of [Ca(2+)](i) responses and that ANG II and bradykinin receptors exist separately on each cell type.
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