These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: 17 beta-estradiol attenuates intimal hyperplasia and macrophage accumulation with a reduction in monocyte chemoattractant protein 1 expression in a vein graft model.
    Author: Ryomoto M, Wolff RA, Tomas JJ, Miyamoto T, Hoch JR.
    Journal: J Vasc Surg; 2002 Sep; 36(3):613-21. PubMed ID: 12218988.
    Abstract:
    OBJECTIVE: Autogenous vein grafts are commonly used for arterial reconstructive procedures. Their success is limited by the development of intimal hyperplasia, a fibroproliferative disease that predisposes the grafts to occlusive stenosis. Our goal was to assess whether 17 beta-estradiol (E(2)) inhibits vein graft intimal hyperplasia coincident with a reduction in monocyte chemoattractant protein 1 (MCP-1) expression and macrophage accumulation. METHOD: Male Lewis rats were implanted with time-release pellets that contained 0.5 mg E(2) (E5 group) or placebo (PL group). Epigastric vein to common femoral artery interposition grafts were harvested at 2, 4, 8, and 12 weeks after surgery. We assessed macrophage/monocytes numbers, proliferating cell nuclear antigen, MCP-1, and transforming growth factor-beta1 with use of immunohistochemistry. MCP-1 message expression was quantified by real-time polymerase chain reaction. RESULTS: The time-release pellets raised the serum E(2) level to greater than 250 pg/mL on the day of surgery. Serum E(2) level declined to 43 +/- 13 pg/mL by 4 weeks and to baseline by 6 weeks. We found that the neointimal area ratio was reduced significantly in the E5 group at 2 and 4 weeks (45%, P <.05, and 68%, P < 0.05, respectively) relative to that in the PL group. The number of proliferating cells was reduced in the E5 group. There was a significant attenuation of MCP-1 expression and of the number of macrophages accumulating in the graft with E(2) treatment. Furthermore, MCP-1 messenger ribonucleic acid expression was also significantly attenuated in the E5 group at 4 weeks when compared to the PL group. There was no significant difference between the two groups in the expression of transforming growth factor-beta1. CONCLUSIONS: E(2) treatment reduces vein-graft intimal hyperplasia coincident with a reduction in MCP-1 expression, macrophage accumulation, and cell proliferation.
    [Abstract] [Full Text] [Related] [New Search]