These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Studies on the Expression of the 28 kD Glutathione S-transferase Gene from Schistosoma japonicum in the Silkworm (Bombyx mori) Larvae and Insect Cells.
    Author: Tian E, Yang GZ, Cai YM, Shi FH, Wu XF.
    Journal: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai); 1997; 29(1):33-39. PubMed ID: 12219234.
    Abstract:
    The gene of the 28 kD glutathione S-transferase (GST) from the Chinese strain of Schistosoma japonicum had been expressed in the silkworm (Bombyx mori) cells and larvae by Bombyx mori nuclear polyhedrosis virus (BmNPV) vector which had been modified. The GST gene was inserted into the right position of the BmNPV genome as identified by Southern hybridization. The product was a 28 kD protein, had GST activity and antigenicity. The yield in BmN cells (1x10(6) cells/ml) was 0.77 mg and more than 5 mg in a silkworm larvae. All the results showed that it is possible to develop the GST expressed in the silkworm larvae to a new kind of genetic engineered schistosomiasis vaccine.
    [Abstract] [Full Text] [Related] [New Search]